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Miltenyi Biotec
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Jackson Immuno
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BPS Bioscience
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Wanleibio
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2026-07
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Novus Biologicals
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2026-07
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Bio X Cell
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Cell Signaling Technology Inc
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Journal: Science Advances
Article Title: Targeting mitocytosis potentiates mitochondria drug delivery for antimetastasis therapy
doi: 10.1126/sciadv.aec7150
Figure Lengend Snippet: ( A and B ) Particle size (A) and zeta potential (B) of tumor cell membrane (T), mitochondrial membrane (M), and hybrid membrane (Hyb; tumor cell membrane:mitochondrial membrane = 1:1). ( C ) SDS-PAGE of T, M, Hyb, and hybrid membrane–coated nanoparticles (Hyb-NPs). ( D ) WB analysis of mitochondrial membrane characteristic protein (MFN-2) and tumor membrane characteristic protein (CD44). ( E ) Illustration of the preparation of various PEG-modified or membrane-coated nanoparticles. Created in BioRender. Mahaha, M. (2026) https://BioRender.com/s1en2tw . ( F and G ) Particle size distribution and TEM images of TL/RP-NPs (F) and TL/RH-NPs (G). ( H ) Stability of TL/RP-NPs and TL/RH-NPs at 37°C within 24 hours in PBS and 10% serum. ( I ) Cumulative release of TPP-LND from TL/RP-NPs or TL/RH-NPs at 37°C within 24 hours in PBS. ( J ) 4T1 cell viability after treatment with TPP-LND, TL/RP-NPs, TL/T-NPs, TL/RT-NPs, or TL/RH-NPs for 24 hours. ( K to M ) Mitochondria uptake of TL/RP-NPs, TL/RT-NPs, or TL/RH-NPs in whole mitochondria (K), IMM plus Matrix (L), and outer mitochondrial membrane (OMM) plus intermembrane space (IMS) (M). All results were presented as means ± SD, n = 3. NS indicated no significance. T, tumor cell membrane; M, mitochondrial membrane; Hyb, hybrid membrane.
Article Snippet:
Techniques: Zeta Potential Analyzer, Membrane, SDS Page, Modification
Journal: Bio-protocol
Article Title: Denaturing SUMO Immunoprecipitation From Mitotic Cells
doi: 10.21769/BioProtoc.5648
Figure Lengend Snippet: The blot displays Cyclin B1 levels in lysates made from either an asynchronous cell population (Async) or cells treated with 100 ng/mL nocodazole for 16 h and harvested via mitotic shake-off (Mitotic). α Tubulin and Histone H3 are included as loading controls. This representative image was obtained from one biological repeat.
Article Snippet: RANGAP1 polyclonal antibody (Invitrogen, catalog number: PA5-92435) 39. (Optional) Pierce TM 660 nm protein assay (ThermoFisher Scientific, catalog number: 22660) 40. (Optional) ionic detergent compatibility reagent (ThermoFisher Scientific, catalog number: 22663) 41. (Optional) Cyclin B1 antibody (Cell Signaling, catalog number: 12231) 42. (
Techniques: